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How to use

Step 1. Download the appropriate color channels set of benchmark images.

Step 2. Open this set of images in the quantitative colocalization analysis software you are using and calculate the values of colocalization coefficients on them.

Step 3. By comparing the values of coefficients on your images with the values of coefficients on pre-defined images, select the closest benchmark image and use its value of colocalization to describe your results.

See examples below:

If you have any questions, check FAQ first before contacting us.

NOTE: The benchmark images were created using BioSim software as described previously. They simulate fluorescence microscopy images with a sampling rate of 4 pixels per resolution element. If the optical resolution is 200 nm, then the benchmark images are corresponding to images with a pixel size of 50 nm. All images are entirely background noise and out-of-focus fluorescence free. The size of images is 1024x1024 pixels.

Image sets

Set 1 (red/green channels)

Name

Colocalization

Type

Format

Size

Download

CBS001RGM

0%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS002RGM

10%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS003RGM

20%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS004RGM

30%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS005RGM

40%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS006RGM

50%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS007RGM

60%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS008RGM

70%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS009RGM

80%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS010RGM

90%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS001RGM-CBS010RGM

0-90%

Merged images

Lossless TIFFs

15.7 MB zip

Download

Use this set if you analyze colocalization on merged red/green image channels.

Set 2 (red/blue channels)

Name

Colocalization

Type

Format

Size

Download

CBS001RBM

0%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS002RBM

10%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS003RBM

20%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS004RBM

30%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS005RBM

40%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS006RBM

50%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS007RBM

60%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS008RBM

70%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS009RBM

80%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS010RBM

90%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS001RBM-CBS010RBM

0-90%

Merged images

Lossless TIFFs

15.7 MB zip

Download

Use this set if you analyze colocalization on merged red/blue image channels.

Set 3 (green/blue channels)

Name

Colocalization

Type

Format

Size

Download

CBS001GBM

0%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS002GBM

10%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS003GBM

20%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS004GBM

30%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS005GBM

40%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS006GBM

50%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS007GBM

60%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS008GBM

70%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS009GBM

80%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS010RBM

90%

Merged image

Lossless TIFF

1.7 MB zip

Download

CBS001GBM-CBS010GBM

0-90%

Merged images

Lossless TIFFs

15.7 MB zip

Download

Use this set if you analyze colocalization on merged green/blue image channels.

Set 4 (single channels)

Name

Colocalization

Type

Format

Size

Download

CBS001R

0%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS001G

0%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS001B

0%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS002R

10%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS002G

10%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS002B

10%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS003R

20%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS003G

20%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS003B

20%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS004R

30%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS004G

30%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS004B

30%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS005R

40%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS005G

40%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS005B

40%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS006R

50%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS006G

50%

Single channel image

Lossless TIFF

0.7 MB zip

Download

CBS006B

50%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS007R

60%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS007G

60%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS007B

60%

Single channel image

Lossless TIFF

0.7 MB zip

Download

CBS008R

70%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS008G

70%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS008B

70%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS009R

80%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS009G

80%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS009B

80%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

CBS010R

90%

Red channel image

Lossless TIFF

0.7 MB zip

Download

CBS010G

90%

Green channel image

Lossless TIFF

0.7 MB zip

Download

CBS010B

90%

Blue channel image

Lossless TIFF

0.7 MB zip

Download

Whole set

0-90%

Single channel images

Lossless TIFFs

19.9 MB zip

Download

Use this set if you analyze colocalization on pairs of single channel images.

Examples

Example 001

Example0012x

Image ID

CBSE001

Image Info

Bsep + Mrp2 in rat liver

Channels

Red/Green

Pearson's (Rr)

0.68

Manders' (R)

0.83

Closest benchmark

CBS008RGM (70%)

Remarks

High degree of colocalization of Bsep and Mrp2 at the bile canaliculi suggests their involvement in the regulation of canalicular transport.

Submitted by Dr. V. Zinchuk, Kochi University, Japan. Preview of the original image is shown.

Example 002

Example0022x

Image ID

CBSE002

Image Info

Parvalbumin + spectrin in rat brain

Channels

Red/Green

Pearson's (Rr)

0.21

Manders' (R)

0.63

Closest benchmark

CBS004RGM (30%)

Remarks

Lack of significant functional similarities between parvalbumin and spectrin in neurons is indicated by their low degree of colocalization.

Submitted by Dr. O. Grossenbacher, Fribourg University, Switzerland. Preview of the original image is shown.

Example 003

Example0032x

Image ID

CBSE003

Image Info

Calcium channels subunits and ryanodine receptors in rat heart

Channels

Red/Green

Pearson's (Rr)

0.61

Manders' (R)

0.71

Closest benchmark

CBS007RGM (60%)

Remarks

Strong colocalization between calcium channels and ryanodine receptors, as indicated by the indices, is essential in cardiac excitation-contraction coupling.

Submitted by Dr. Y. Wu, UCLA, U.S.A. Preview of the original image is shown.

Recommended citation

"Image set CBS001RGM-CBS010RGM from the Colocalization Benchmark Source (www.colocalization-benchmark.com) was used to validate colocalization."

License

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Copyright 2012-2014 Colocalization Benchmark Source. All rights reserved.